NAD+ reconstitution calculator
NAD+ reconstitution calculator. Enter vial size and bacteriostatic water to get concentration and the insulin syringe units for any amount.
| Amount | Units (U-100) | mL |
|---|---|---|
| 50 mcg | 0 | 0 |
| 100 mcg | 0 | 0 |
| 250 mcg | 0.1 | 0.001 |
| 500 mcg | 0.1 | 0.001 |
| 1 mg | 0.2 | 0.002 |
| 2 mg | 0.4 | 0.004 |
| 2.5 mg | 0.5 | 0.005 |
| 5 mg | 1 | 0.01 |
How the math works
Concentration is the peptide in the vial divided by the water you add. A 10 mg vial with 2 mL of bacteriostatic water holds 5 mg (5,000 mcg) per mL. On a U-100 insulin syringe, 1 mL is 100 units, so each unit carries 50 mcg. To draw 250 mcg you would pull 5 units.
The amount field is blank on purpose. This site does not suggest amounts. The NAD+ page lists what published studies used.
Doses used in published research
What investigators actually administered, as reported in the cited study. This is a record of the literature, not a recommendation.
| Study | Population | Regimen |
|---|---|---|
| Grant et al., Front Aging Neurosci 2019 | Healthy men aged 30 to 55 (8 NAD+, 3 saline) | 750 mg NAD+ intravenous over 6 hours (about 3 micromol/min), single infusion |
| Reyna et al., Front Aging 2026 (retrospective) | 6 adults at a US wellness clinic | 500 mg NAD+ in 500 mL saline intravenous, 4 consecutive days, rate set by tolerance |
| Martens et al., Nat Commun 2018 (precursor NR) | Healthy adults aged 55 to 79 | Nicotinamide riboside 500 mg orally twice daily, 6 weeks, crossover with placebo |
| Yoshino et al., Science 2021 (precursor NMN) | Postmenopausal women with prediabetes | Nicotinamide mononucleotide 250 mg orally daily, 10 weeks |
New to this? Read Peptides 101 and the syringe units chart.
Common questions
How much bacteriostatic water should I add to a NAD+ vial?
There is no single correct volume. More water makes each unit carry less peptide, which makes small amounts easier to measure; less water keeps volumes small. Most people pick a volume that puts their amount between 5 and 50 units on a U-100 syringe.
What does U-100 mean?
U-100 insulin syringes are marked in units where 100 units equals 1 mL. So 10 units is 0.1 mL regardless of what is in the syringe. Peptide calculators use this scale because insulin syringes are what is widely available in Philippine pharmacies.
Does the calculator work for mg and mcg?
Yes. 1 mg equals 1,000 mcg. Switch the unit next to the amount field and the result updates.
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